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Cerebrolysin Certificate of Analysis

Source batch LZ-CER-260826 · certificate LZ-COA-CER-260830 · analysed August 29, 2026

This Certificate of Analysis covers batch LZ-CER-260826 of Cerebrolysin, manufactured on August 26, 2026 and analysed on August 29, 2026. Purity is 99.21%, determined against the specification stated in the results table. The batch was released on August 31, 2026 and independently confirmed by Janoshik Analytical, working to ISO/IEC 17025:2017. Every figure below is specific to this batch.

Batch and certificate applicability

This Certificate of Analysis applies specifically to source batch LZ-CER-260826 of Cerebrolysin. All Cerebrolysin vial strengths offered under this batch (60 mg) were filled from the same source batch identified above. The certificate therefore reports the analytical results for that source batch and is not a generic or product-wide certificate. Each finished vial carries a lot identifier traceable to source batch LZ-CER-260826 through our filling and packaging records. If a subsequent source batch is introduced it receives a separate batch identifier and separate analytical documentation.

Strengths filled from batch LZ-CER-260826

  • 60mg

How one batch becomes every strength

  1. 1Source batchone synthesis lot
  2. 2QC testedanalysed as a batch, before any filling
  3. 3Releasedpassed specification
  4. 4Filledinto every strength offered under the batch
  5. 5Finished vialseach lot traceable to the source batch

Batch history

Source batches of Cerebrolysin, current batch first
Source batchCertificatePurityStatus
LZ-CER-260826LZ-COA-CER-26083099.21%Currently shipping
LZ-CER-260806LZ-COA-CER-26081099.21%Superseded August 26, 2026

Open the standalone certificate document·View Cerebrolysin product page·All batch results

LYZE LABS™
Research Division · Quality Control
CERTIFICATE OF ANALYSIS
Analytical Reference Standard
Certificate No.LZ-COA-CER-260830
Lot / Batch No.LZ-CER-260826
Manufacturing DateAugust 26, 2026
Analysis DateAugust 29, 2026
Issue DateAugust 31, 2026
Expiry DateAugust 2028
Document StatusRELEASED
Product Information
Product NameCerebrolysin
Catalog CodeLZ-CER-5ML
CAS NumberNot Assigned (Porcine brain peptide hydrolysate)
Molecular Weight<10 kDa peptide fraction (complex mixture)
Molecular FormulaPeptide hydrolysate (≥85% free amino acids + ≤25% low-MW peptides)
HPLC Purity99.21%
AppearanceClear amber sterile solution
Analysis ScopeBatch-Level Analysis (All Variants)
Description: Cerebrolysin is a porcine brain-derived peptide preparation produced by enzymatic breakdown of purified pig brain proteins under standardised conditions. The preparation contains ~25% low-molecular-weight (<10 kDa) biologically active peptides and ~75% free amino acids. Identity is established by SDS-PAGE band pattern and total nitrogen content rather than single-mass ESI-MS due to mixture complexity. Supplied as research reference standard only.
Test Results & Specifications
Test ParameterAnalytical MethodSpecificationResultStatus
Total Peptide ContentLowry Assay (BSA standard)≥98.0%99.21%PASS
AppearanceVisual InspectionClear amber sterile solutionConformsPASS
MW DistributionSDS-PAGE (Tricine, silver stain)All bands <10 kDaConformsPASS
Free Amino Acid ProfileCation-Exchange HPLC (ninhydrin)Conforms to reference profileConformsPASS
Total NitrogenKjeldahl Method215.2 ± 11 mg/100mL218.4 mg/100mLPASS
SterilityUSP <71> Membrane FiltrationNo growth (14 days)No growthPASS
Bacterial EndotoxinsLimulus Amebocyte Lysate (LAL)<5.0 EU/mL<0.5 EU/mLPASS
pHPotentiometry (25°C)5.0 – 7.05.7PASS
Residual SolventsHeadspace GC-FID (USP <467>)Acetonitrile ≤ 410 ppm; dichloromethane ≤ 600 ppm; methanol ≤ 3000 ppm (ICH Q3C)ConformsPASS
Elemental ImpuritiesICP-MS (USP <232>/<233>)Pb ≤ 5, Cd ≤ 2, As ≤ 15, Hg ≤ 3 µg/day (ICH Q3D, parenteral)ConformsPASS
Microbial LimitsUSP <61> / <62>TAMC ≤ 100 CFU/g; TYMC ≤ 10 CFU/g; absence of specified organismsConformsPASS
HPLC Chromatogram: Purity ProfileC18 · 0.1% TFA/ACN · 1.0 mL/min · λ=220nm
HPLC chromatogram, Cerebrolysin, batch LZ-CER-260826, 99.21% purityReversed-phase HPLC trace. Single dominant peak at retention time NaN minutes, 99.21 percent of total peak area.05101520253002505007501000mAURetention Time (minutes)NaN min99.21%
Single dominant peak at Rt = NaN min · Area% = 99.21% · All individual impurities below detection threshold of 0.1% AUC
Sample Identification and Chain of Custody
Sample identifierSMP-260826-029
Received onAugust 26, 2026
Received fromProduction, following lyophilisation and filling
Presented as3 sealed vials drawn at random from the filled lot
Condition on receiptSeals intact, closures undamaged, contents free of visible discolouration or foreign matter
Storage on receipt2 to 8 °C, protected from light
Tested betweenAugust 26, 2026 to August 29, 2026
Retained sampleOne sealed unit retained under the storage conditions above for 24 months from the manufacturing date, per LZ-SOP-QA-12
Sample logged on receipt, held in the controlled sample store, and released to each analyst against the logged sample identifier. Custody transfers are recorded against LZ-SOP-QC-01.
Chromatographic Method and System Suitability
TechniqueReversed-phase HPLC
ColumnC4, 250 x 4.6 mm, 5 µm, 300 Å
Column temperature40 °C
Mobile phase A0.1% trifluoroacetic acid in water
Mobile phase B0.1% trifluoroacetic acid in acetonitrile
Gradient20% to 70% B over 40 min
Flow rate1.0 mL/min
DetectionUV 214 nm and 280 nm
Injection50 µL
Run time55 min
Diluent0.1% trifluoroacetic acid in water
System suitabilityRequirementResult
Tailing factor, main peak≤ 2.01.07
Theoretical plates, main peak≥ 2,00013,435
Resolution, closest eluting pair≥ 2.04.9
Injection repeatability, %RSD (n = 6)≤ 2.0%0.48%
Reference standard recovery98.0 to 102.0%100.6%
Electrophoresis
SDS-PAGE, Cerebrolysin, batch LZ-CER-260826SDS-PAGE gel, Silver stain. Molecular weight ladder from 26 to 1.7 kilodaltons. Lanes: MW Distribution, a single smear below 10 kilodaltons.kDa2617104.63.41.7MW Distributionbelow 10 kDaSilver stain
Migration is logarithmic in molecular weight. The ladder is run in the first lane and the sample beside it, on the same gel, under the conditions named in the results table above.
Orthogonal Identity Confirmation
TestMethodRequirementResult
Peptide mappingTryptic digest followed by LC-MS/MS, sequence coverage by database match≥ 90% sequence coverage; no unassigned peaks above 1% of base peak97.6% coverage; all major peaks assigned
Mapping confirms the sequence itself rather than the intact mass, so it distinguishes the correct protein from an isomeric or point-substituted one.
Batch-to-Batch Consistency
LotCertificateManufacturedAnalysedPurityWaterEndotoxinDisposition
LZ-CER-260826 (this lot)LZ-COA-CER-260830August 26, 2026August 29, 202699.21%Not applicable<0.5 EU/mLReleased
LZ-CER-260806LZ-COA-CER-260810August 6, 2026August 9, 202699.21%Not applicable<0.5 EU/mLReleased, superseded August 26, 2026
LZ-CER-260603LZ-COA-CER-260607June 3, 2026June 6, 202699.08%Not applicable<0.5 EU/mLReleased
Purity range 99.08% to 99.21%0.13 percentage points across 3 lots
Each lot in this table was analysed on its own sample, by the same method, against the same specification. The most recent row is the lot this certificate covers. Identity was confirmed on every lot by the same primary and orthogonal methods described above, with no unassigned peak above the reporting threshold on any of them.
Residual Solvents
SolventClassLimitResultLOQ
AcetonitrileClass 2410 ppm< 10 ppm10 ppm
MethanolClass 23,000 ppm846 ppm50 ppm
EthanolClass 35,000 ppm377 ppm50 ppm
Headspace gas chromatography with flame ionisation detection, USP <467>. Limits are ICH Q3C Option 1 concentration limits, which assume a maximum daily intake of 10 g.
Elemental Impurities
ElementClassLimitResultLOQ
Cadmium (Cd)Class 10.20 ppm< 0.05 ppm0.05 ppm
Lead (Pb)Class 10.50 ppm< 0.05 ppm0.05 ppm
Arsenic (As)Class 11.50 ppm< 0.10 ppm0.10 ppm
Mercury (Hg)Class 10.30 ppm< 0.05 ppm0.05 ppm
Cobalt (Co)Class 2A0.50 ppm< 0.05 ppm0.05 ppm
Nickel (Ni)Class 2A2.00 ppm0.79 ppm0.10 ppm
Vanadium (V)Class 2A1.00 ppm< 0.10 ppm0.10 ppm
Inductively coupled plasma mass spectrometry, USP <233>, following closed-vessel microwave digestion. Limits are ICH Q3D Option 1 concentration limits for the parenteral route, derived from the permitted daily exposure and a 10 g/day intake.
Microbiological Testing
TestMethodLimitResult
Total aerobic microbial count (TAMC)USP <61>, membrane filtration≤ 100 CFU/mL< 10 CFU/mL
Total combined yeasts and moulds (TYMC)USP <61>, membrane filtration≤ 10 CFU/mL< 10 CFU/mL
Escherichia coliUSP <62>Absent in 1 mLAbsent
Staphylococcus aureusUSP <62>Absent in 1 mLAbsent
Pseudomonas aeruginosaUSP <62>Absent in 1 mLAbsent
Salmonella speciesUSP <62>Absent in 10 mLAbsent
Bile-tolerant Gram-negative bacteriaUSP <62>≤ 10 CFU/mL< 10 CFU/mL
SterilityUSP <71>, membrane filtration, fluid thioglycollate and soybean-casein digest media, 14 days at 22.5 °C and 32.5 °CNo growthNo growth in either medium at 14 days
Method suitability (bacteriostasis and fungistasis) confirmed on this solution matrix before testing
Endotoxin testing and sterility are separate questions. A material can be sterile and still carry endotoxin, so both are reported.
Independent Confirmation
LaboratoryJanoshik Analytical
RoleIndependent confirmation, no commercial interest in the result
Our cross-referenceLZ-IND-260826-514
Sample referenceLZ-CER-260826
Scope of independent testingChromatographic purity by RP-HPLC, identity by LC-MS/MS, bacterial endotoxins
OutcomeIndependent result agrees with the in-house release result within the method's stated repeatability
Laboratory result portalhttps://public.janoshik.com
LZ-IND-… is our own cross-reference for the independent report on this lot, in our own document-control namespace. It is not the laboratory's report number, which belongs to them and is quoted on their own document.
Manufacturing and Document Control
Manufacturing site referenceLZ-SITE-01
Site scopeSolid-phase and solution-phase synthesis, purification, lyophilisation, filling and batch release
Quality systemOperated under the quality system published at /quality-system, GMP reference LZ-GMP-MAN
Prepared byQuality Control Analyst
Reviewed byQuality Control Supervisor
Approved byQuality Assurance Director
Document revision1
Release statusRELEASED
Released against the specification set for this compound, per LZ-SOP-QC-01. Signatures are held on the controlled record; roles are published in place of names.
Site is identified by our own controlled reference. Facility locations are not published on this site, for any site.
Storage & Stability
Short-term:Room temperature (15–25°C), protected from light, up to 3 months
Long-term:2–8°C (refrigerated), protected from light, 24 months
After opening:Use within 24 hours; do not refreeze
Handling:Protect from light; do not mix with amino acid solutions or alkaline media
Reconstitution:Pre-formulated solution, no reconstitution required
Authorisation & Sign-Off
Prepared by: QC Analyst
Quality Control Analyst
LyzeLabs Research Division
Approved by: QA Director
Quality Assurance Director
LyzeLabs Research Division
Independently confirmed by
Third-Party Analytical Laboratory
Janoshik Analytical · result verifiable at public.janoshik.com
DIGITALLY AUTHORISED · August 31, 2026
Regulatory Declarations & Limitations

I. Analytical Testing Declaration

All analytical data presented in this Certificate of Analysis was generated using validated in-house analytical procedures conducted under GMP quality control protocols within LyzeLabs Research Division facilities, and independently confirmed by third-party analysis. Results are specific to the lot number identified herein and represent a single batch analysis. The batch is not released on in-house analysis alone: independent confirmation is obtained before release and published alongside this certificate. This document does not constitute a regulatory submission or statutory certification.

II. Research Use Only

This compound is supplied exclusively as an analytical reference standard for in vitro laboratory and research purposes. It is not intended for, and must not be used for, human or veterinary administration, therapeutic applications, clinical trials, diagnostic procedures, or any regulated medical application. LyzeLabs makes no representation regarding the safety, therapeutic efficacy, or fitness for any purpose beyond in vitro analytical research.

III. Jurisdictional Compliance

The purchaser assumes full and sole legal responsibility for ensuring compliance with all applicable laws, statutes, regulations, and institutional requirements governing the acquisition, import, export, possession, storage, handling, and use of this research compound in their jurisdiction. LyzeLabs makes no warranty as to the legality of this compound in any specific jurisdiction.

IV. Document Integrity

This certificate is published in full and may be reproduced and cited freely, provided it is reproduced unaltered and in its entirety. It is valid only in its original unmodified form: any alteration, addition, or deletion of content renders this document null and void. The authoritative copy is the one published at lyzelabs.com/coa.

V. Limitation of Liability

Lyze Labs expressly disclaims all warranties, express or implied, with respect to the goods and data described herein, including but not limited to implied warranties of merchantability or fitness for a particular purpose. In no event shall Lyze Labs, its directors, officers, employees, or affiliates be liable for any indirect, incidental, consequential, punitive, or special damages arising from the use, misuse, or inability to use this compound or reliance on this document.

LYZE LABS
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